In January 2000, the Health Department of the Municipality of Belo Horizonte, State of Minas Gerais, Brazil, carried out a seroepidemiologic study – Dengue Seroprevalence Survey in Belo Horizonte (ISDBH) – in order to determine and quantify the seroprevalence of dengue virus infection in the city. This article presents the seroprevalence of dengue infection in the surveyed Sanitary Districts, for a random sample of 627 individuals. The total infection prevalence reached 23.3%. Among the participants, 17.9% were seropositive for DENV-1 and DENV-2, 4.6% only for DENV-1 and 0.8% only for DENV-2. Seropositive individuals, when compared to seronegative persons, were more likely to reside in Sanitary Districts Leste and Venda Nova; had never moved out of their houses or neighborhoods and lived in horizontal residential buildings. Low income was associated with high prevalence rates, horizontal residential buildings with vector infestation and the lack of spatial mobility of participants with high infection rates.