Advanced topics in Molecular Medicine - MED 821

30 hours/ 2 credits

Ementa:

Seminars addressing newly acquired knowledge on relevant topics of Molecular Medicine or related subjects and/or practical course on current methods, enabling different approaches to important scientific issues of interest to teachers and students of the graduate program.

References:

Given at the start of each semester.

Cellular Signaling - MED 815


60 hours/ 4 credits

Ementa:

Discussion of basic concepts related to inter-and intracellular signaling, such as drug-receptor interaction, receptor coupled to ion channels or G-protein regulation by phosphorylation, kinase cascades. Thereafter, students present seminars on recent work to be discussed by the group, comprising critical analysis of techniques used, results and conclusions.


References:

Atlas D (2013) The voltage-gated calcium channel functions as the molecular switch of synaptic transmission. Annu Rev Biochem. Jan 17. [Epub ahead of print]

Clapham DE (2007) Calcium signaling. Cell 131: 1047-1058.

Corbalán-García S, Gómez-Fernández JC (2006) Protein kinase C regulatory domains: the art of decoding many different signals in membranes. Biochim Biophys Acta 1761: 633-654

Fukada T, Yamasaki S, Nishida K, Murakami M, Hirano T (2011) Zinc homeostasis and signaling in health and diseases: Zinc signaling. J Biol Inorg Chem 16:1123-1134

Johnson DB, Sosman JA (2013. Update on the Targeted Therapy of Melanoma. Curr Treat Options Oncol. Feb 19. [Epub ahead of print]

Kenakin T, Christopoulos A (2012) Signalling bias in new drug discovery: detection, quantification and therapeutic impact. Nat Rev Drug Discov 12:205-516

Lania AG, Mantovani G, Spada A (2006) Mechanisms of disease: Mutations of G proteins and G-protein-coupled receptors in endocrine diseases. Nat Clin Pract Endocrinol Metab 2: 681-693

Lima MH, Caricilli AM, de Abreu LL, Araújo EP, Pelegrinelli FF, Thirone AC, Tsukumo DM, Pessoa AF, dos Santos MF, de Moraes MA, Carvalheira JB, Velloso LA, Saad MJ (2012) Topical insulin accelerates wound healing in diabetes by enhancing the AKT and ERK pathways: a double-blind placebo-controlled clinical trial. PLoS One 7:e36974

Niehrs C (2012) The complex world of WNT receptor signaling. Nature Rev Mol Cell Biology 13:767-779

Xing M (2013) Molecular pathogenesis and mechanisms of thyroid cancer. Nat Rev Cancer 13:184-199


Laboratory Methods - MED 817


60 hours/ 4 credits

Ementa:

The course aims to introduce students to basic techniques routinely used in research laboratories. Thus, it will be focused on practical and theoretical classes of: weighing, measuring and calibration of volumetric material, titration, colorimetry, centrifugation, cellular fractionation, chromatography, fluorimetry.


References:

Braga GL, Collins CH. Introdução a Métodos Cromatográficos. Ed. Unicamp, Campinas.

de Duve C (1971) Tissue fractionation: past and present. J Cell Biol 50: 20D-55D.

Mendham J, Denney RC, Barnes JD, Thomas MJK (2000) Vogel’s Quantitative Chemical Analysis. 6th Edition, Prentice Hall, UK.

Nicholls D (1994) Proteins, transmitters and synapses. Blackwell Scientific. 1st edition.

Snell FD, Snell CT. Colorimetric methods of analysis. Volume IV A. van Wostrand Reinhold Co, New York.

Stalil E. Thin Layer Chromatography. Spring Verlag, Amsterdam.

Turner AJ, Bachelard HS (1997) Neurochemistry – a practical approach. Oxford University Press, 2nd edition.

Wilson K, Goulding K (1986) A biologist’s guide to principles and techniques of practical biochemistry. E. Arnold. 3rd edition.


Molecular Medicine I - MED 813


60 hours/ 4 credits

Ementa:

Introduction of general concepts of molecular biology, biochemistry and general genetics.


References:

Aparicio S, Carlos C (2013) The implications of clonal genome evolution for cancer Medicine. New Engl J Med 368:842-51

Ausubel FM (2002) Short Protocols in Molecular Biology, Canadá, Wiley

Balic M, Williams A, Lin H, Datar R, Cote RJ (2013) Circulating tumor cells: from bench to bedside. Annu Rev Med 64:31–44

Ghosh S, Matsuoka Y, Asai Y, Hsin K-Y, Kitano H (2011) Software for systems biology: from tools to integrated platforms. Nature Rev Genet 12:821-832

Hames BD, Higgins SJ (1995) Gene Probes 2. Oxford University Press, Oxford.

Hoban S, Bertorelle G, Gaggiotti OE (2012) Computer simulations: tools for population and evolutionary genetics. Nature Rev Genet 13:110-122

Strachan T, Read AP (2004) Human Molecular Genetics 3. Garland Science, New York.

Tenesa A, Haley CS (2013) The heritability of human disease: estimation, uses and abuses. Nature Reviews Genetics 14, 139-149

Wheeler HE, Maitland ML, Dolan ME, Cox NJ, Ratain MJ (2013) Cancer pharmacogenomics: strategies and challenges. Nature Rev Genet 14:23-34


MED 816 - Medicina Molecular I


60 hours/ 4 credits

Ementa:

Estudo dos conceitos gerais de bioquímica, biologia molecular e genética.


Bibliografia:

Aparicio S, Carlos C (2013) The implications of clonal genome evolution for cancer Medicine. New Engl J Med 368:842-851

Ausubel FM (2002) Short Protocols in Molecular Biology, Canadá, Wiley

Balic M, Williams A, Lin H, Datar R, Cote RJ (2013) Circulating tumor cells: from bench to bedside. Annu Rev Med 64:31–44

Ghosh S, Matsuoka Y, Asai Y, Hsin K-Y, Kitano H (2011) Software for systems biology: from tools to integrated platforms. Nature Rev Genet 12:821-832

Hames BD, Higgins SJ (1995) Gene Probes 2. Oxford University Press, Oxford.

Hoban S, Bertorelle G, Gaggiotti OE (2012) Computer simulations: tools for population and evolutionary genetics. Nature Rev Genet 13:110-122

Strachan T, Read AP (2004) Human Molecular Genetics 3. Garland Science, New York.

Tenesa A, Haley CS (2013) The heritability of human disease: estimation, uses and abuses. Nature Rev Genet 14, 139-149

Wheeler HE, Maitland ML, Dolan ME, Cox NJ, Ratain MJ (2013) Cancer pharmacogenomics: strategies and challenges. Nature Rev Genet 14:23-34


MED 817 - Medicina Molecular II


60 hours/ 4 credits

Ementa:

Discussão de aspectos relacionados à genômica, farmacogenômica e farmacogenética. Os estudantes apresentam seminários sobre temas atuais.


Bibliografia (exemplos):

Blanpain C (2013) Tracing the cellular origin of cancer. Nature Cell Biology 15: 126–134

Franke B, Faraone SV, Asherson P, Buitelaar J, Bau CHD, Ramos-Quiroga JA, Mick E, Grevet EH, Johansson S, Haavik J, Lesch K-P, Cormand B, Reif A on behalf of the International Multicentre persistent ADHD CollaboraTion (IMpACT) (2012) The genetics of attention deficit/hyperactivity disorder in adults, a review. Mol Psychiatry 17:960-987

Frank M (2013) Open but not free – publishing in the 21st century. N Engl J Med 368:787-789

Muller PAJ, Vousden KH (2012) p53 mutations in cancer. Nature Cell Biology 15:2-8

Pena SD, Di Pietro G, Fuchshuber-Moraes M, Genro JP, Hutz MH, Kehdy Fde S, Kohlrausch F, Magno LA, Montenegro RC, Moraes MO, de Moraes ME, de Moraes MR, Ojopi EB, Perini JA, Racciopi C, Ribeiro-Dos-Santos AK, Rios-Santos F, Romano-Silva MA, Sortica VA, Suarez-Kurtz G (2011) The genomic ancestry of individuals from different geographical regions of Brazil is more uniform than expected. PLoS One 6:e17063

Smith ZD, Meissner A (2013) DNA methylation: roles in mammalian development. Nature Rev Genet 14: 204-220

Willis MS, Patterson C (2013) Proteotoxicity and cardiac dysfunction – Alzheimer’s disease of the heart? N Engl J Med 368: 455-464


Molecular Medicine II - MED 814


60 hours/ 4 credits

Ementa:

Discussion of aspects related to genomics, pharmacogenetics and pharmacogenomics. Students present seminars with recent published data in relevant journals


References:

Blanpain C (2013) Tracing the cellular origin of cancer. Nature Cell Biology 15: 126–134

Franke B, Faraone SV, Asherson P, Buitelaar J, Bau CHD, Ramos-Quiroga JA, Mick E, Grevet EH, Johansson S, Haavik J, Lesch K-P, Cormand B, Reif A on behalf of the International Multicentre persistent ADHD CollaboraTion (IMpACT) (2012) The genetics of attention deficit/hyperactivity disorder in adults, a review. Mol Psychiatry 17:960-987

Frank M (2013) Open but not free – publishing in the 21st century. N Engl J Med 368:787-789

Muller PAJ, Vousden KH (2012) p53 mutations in cancer. Nature Cell Biology 15:2-8

Pena SD, Di Pietro G, Fuchshuber-Moraes M, Genro JP, Hutz MH, Kehdy Fde S, Kohlrausch F, Magno LA, Montenegro RC, Moraes MO, de Moraes ME, de Moraes MR, Ojopi EB, Perini JA, Racciopi C, Ribeiro-Dos-Santos AK, Rios-Santos F, Romano-Silva MA, Sortica VA, Suarez-Kurtz G (2011) The genomic ancestry of individuals from different geographical regions of Brazil is more uniform than expected. PLoS One 6:e17063

Zachary D. Smith, ZD, Meissner A (2013) DNA methylation: roles in mammalian development. Nature Rev Genet 14, 204-220

Willis MS, Patterson C (2013) Proteotoxicity and cardiac dysfunction – Alzheimer’s disease of the heart? N Engl J Med 368: 455-464


Other papers given at the start of each semester.


Neuropharmacology: molecular aspects - MED 818


45 hours/ 3 credits

Ementa:

Introduction to molecular aspects of pre-synaptic neurotransmission. Mechanisms of action of drugs and toxins that affect the nervous system. The course will focus on the role of neurotransmitters and the regulation of synaptic function by drugs. There will be discussion of scientific papers and demonstration of techniques used to study mechanisms controlling neurotransmitter release.


References:

Brennand KJ, Simone A, Tran N, Gage FH (2012) Modeling psychiatric disorders at the cellular and network levels. Mol Psychiatry 17:1239-1253

Feuerstein TJ (2008) Presynaptic receptors for dopamine, histamine, and serotonin. Handb Exp Pharmacol 184: 289-233

Fink KB, Gothert M (2007) 5-HT receptor regulation of neurotransmitter release. Pharmacol Rev 59: 360-417

Hanson ND, Owens MJ, Nemeroff CB (2011) Depression, antidepressants, and neurogenesis: a critical reappraisal. Neuropsychopharmacology 36:2589–2602

Henry A, Kisicki MD, Varley C (2012) Efficacy and safety of antidepressant drug treatment in children and adolescents. Mol Psychiatry 17:1186-1193

Jakobsson J, Zetterberg H, Blennow K, Ekman CJJ, Johansson AJM, Landén M (2013) Altered concentrations of amyloid precursor protein metabolites in the cerebrospinal fluid of patients with bipolar disorder. Neuropsychopharmacology 38:664–672

McNeil BD, Wu LG (2009) Location matters: synaptotagmin helps place vesicles near calcium channels. Neuron 63: 419-421.

Matveev V, Bertram R, Sherman A (2009) Ca2+ current versus Ca2+ channel cooperativity of exocytosis. J Neurosci 29: 12196-12209.

Neher E, Sakaba T (2008) Multiple roles of calcium ions in the regulation of neurotransmitter release. Neuron 59: 861-872

Rizo J, Rosenmund C (2008) Synaptic vesicle fusion. Nat Struct Mol Biol 15: 665-674

Selvaraj S, Faulkner P, Mouchlianitis E, Roiser JP, Howes (2012) Effects of citalopram on serotonin neurotransmission. Mol Psychiatry 17:1143

Zhang J, Narr KL, Woods RP, Phillips OR, Alger JR, Espinoza RT (2013) Glutamate normalization with ECT treatment response in major depression. Mol Psychiatry 18, 268-270


Pharmacokinetics – MED 812


60 hours/ 4 credits

Ementa:

Studies on absorption, distribution and elimination of drugs as well pharmacokinetic parameters for the following uses:

a) establishment of appropriate dosage regimens;

b) assessment of newly developed drugs intended for human use.


References:

Clark B, Smith DA (1993) An introduction to pharmacokinetics. Blackwell Scientific Publications, Oxford.

Brunton L, Lazo J, Parker K (2005) Goodman & Gilman The Pharmacological Basis of Therapeutics, 11th ed., McGraw-Hill, New York.

De Marco L, Boson WL (1998) Farmacocinética e biodisponibilidade. (In: Terapêutica Clinica (Rocha MOC, Pedroso ERP, Fonseca JGM, Silva OA, eds.), Guanabara Koogan.

DiPiro JT, Spruill WJ, Blouin RA, Pruemer JM, Wade WE (2005) Concepts in clinical pharmacokinetics. 4th ed, Bethesda, American Society of Health-Pharmacists.

Shargel L, Yu ABC (1985) Applied biopharmaceutics and pharmacokinetics. Appleton-Century Crofts, Norwalk.


Seminars I - MED 819

15 hours/ 1 credits

Ementa:

Seminars given by graduate students and visiting scientists, discussing recent data related to his/her work and, in addition, work being performed elsewhere. Attended by the Faculty. Attendance is mandatory to all graduate students and optional to undergraduates working in the labs. Each student prepares and delivers a seminar based on the literature and discusses presentations by other students.

Seminars II - MED 820

15 hours/ 1 credits

Ementa:

Each student prepares and delivers a seminar based on the literature and discusses presentations by other students, as part of their training teaching skills. Seminars are mandatory to all graduate students who receive grants from CAPES (according to CAPES Ordinance nº 64, 18 de novembro 2002). Every other semester, these seminars are in English.